Characterization of bovine ileal epithelial cell line for lectin binding, susceptibility to enteric pathogens, and TLR mediated immune responses
نویسندگان
چکیده
In this study, primary and immortalized bovine intestinal epithelial cells (BIECs) were characterized for the expression of surface carbohydrate moieties. Primary BIEC-c4 showed staining greater than 90 % 16 lectins but less 50 four lectins. Immortalized BIECs significantly different lectin binding profile few compared to cells. studied infectivity E. coli, Salmonella enterica, rotavirus, coronavirus, viral diarrhea virus. Bovine strain coli B41 adhered strains S. Dublin Mbandaka strong cell invasion. susceptible rotavirus. LPS stimulation upregulated IL-10, IL-8, IL-6 Poly I:C TLR 8 9 expression. This study provides important knowledge on glycoconjugate immune responses bacterial pathogens or ligands.
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ژورنال
عنوان ژورنال: Comparative Immunology Microbiology and Infectious Diseases
سال: 2021
ISSN: ['1878-1667', '0147-9571']
DOI: https://doi.org/10.1016/j.cimid.2020.101581